產(chǎn)品英文名稱：Rat IFN-γ Flex Set
產(chǎn)品中文名稱：大鼠干擾素-γ 伸縮裝置
Rat IFN-γ Flex Set 產(chǎn)品介紹
The BD™ CBA Rat IFN-γ Flex Set is a bead-based immunoassay capable of measuring rat interferon-γ (IFN-γ) in serum and cell culture supernatant samples. Rat reactivity was determined by testing samples with the BD CBA Rat IFN-γ Flex Set. The biology and function of IFN-γ has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266 or 558267).
This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 ml polypropylene tube for reconstitution. When reconstituted in 4.0 ml Assay Diluent, the standard has a protein concentration of 10,000 pg/ml. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect PE Detection Reagent from prolonged exposure to light.
大鼠干擾素-γ Flex set 的成分
•Capture beads
•detection reagent,
•standards
大鼠干擾素-γ Flex set 的優(yōu)越性
•高靈敏度
•高通量，高含量的分析
•最小體積的生物材料的要求
•適用于冷凍/解凍的生物樣品 •與其他檢驗(yàn)方法兼容。例如，細(xì)胞分析，BD™ ELISPOT可以轉(zhuǎn)移克隆，增殖實(shí)驗(yàn)，流式細(xì)胞術(shù)或其他分析方法。
Rat IFN-γ Flex Set 推薦檢驗(yàn)步驟
The BD CBA Rat IFN-γ Flex Set must be used in conjunction with a BD CBA Mouse/Rat Soluble Protein
Master Buffer Kit (Cat. No. 558266, 100 tests, or 558267, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488).Detailed instructions on the use of this product can be found in the manual for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Rat IFN-γ Flex Set will be 10,000 pg/ml.
When multiplexing the BD CBA Rat IFN-γ Flex Set assay with the BD CBA Rat IL-4 Flex Set assay (Cat. No. 558307), significantly higher background will be seen in the rat IL-4 assay. This increased background, while reducing the sensitivity of the rat IL-4 assay, will not effect the quantitation of rat IFN-γ or IL-4 in any other way.
The BD CBA Rat IFN-γ Flex Set should not be used in the same assay well with any non-BD CBA Rat Soluble Protein Flex Set reagents (such as BD CBA Human or Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Rat Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/flexset
大鼠干擾素-γ Flex set 使用注意事項(xiàng)
1. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
2. ProClin is a trademark of Rohm and Haas Company.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
